Tuesday, August 25, 2020
Once you have read the chapter or assignment, you have to decide how Essay - 1
When you have perused the section or task, you need to conclude how to expound on it - Essay Example ives a view normally overlooked, for the most part by strategy advocates.â Just like some other open arrangement, the detail is in execution, and usage more regularly appears to be more earnestly and complex than predicted by those in favor.â If the feasible issues are not anticipated and tended to the will be a precipitous issues during the time spent execution that may wreck everything else.â Despite incrementalist hypothesis preferring getting some approach embraced on the suspicion that amendment will accompany time, early disappointment can hinders authenticity and work towards blocking future activities (Jacobs 2002).â simultaneously, estimating the achievement of any strategy against the hopeful and expanded cases of its promoters gives the doubter a chose and beguiling bit of leeway (Jacobs 2002). My study is that there are better methods of defeating the legitimate and down to earth issues of authorization and usage than those introduced by Jacobs. Jacobs investigates the degree of gun proprietorship and the profoundly established situation of weapons in American culture.â He anyway erroneously puts forth the defense that any administrative control must fight with both the immense existing populace of guns and the settled in political and social help for singular firearm possession. This isn't accurate in light of the fact that to bring everybody into board isn't such a simpler thing accomplished, you can't fulfill everybody. In addition, Jacobs featured on both the Second Amendment and federalism as boundary to new regulation.â This part presents delivered an all around considered investigation usage hindrances explicit to weapons and basic to general administrative policy.â His study of administrative execution briefly notes, ââ¬Å"If an administrative plan isn't authorized, it loses credibility.â⬠â This is something that all execution supporters should take into their brains. Despite the fact that Jacobs made an excellent showing to looking onto execution challenges he neglects to detail the conceiving solutions.â In the section distrust is
Saturday, August 22, 2020
Descriptive- the Book I Want Essay Example for Free
Expressive the Book I Want Essay There are minutes during the day when there is simply an excess of clamor. Background noise from the TV in the corner. The high pitch buzz of exciting music blasts from earbuds embedded into the ears of somebody close by. Indeed, even the resolute clickity-clatter of fingers over a PC console appear to add to the whirlwind of traffic previously flushed into my brain, through my overpowered ears. For me, there is one second in my day that calm is cherished. At the point when I can no longer take it, I getaway to a physical book shop and treat myself to a hardback book. At the point when I stroll in, I am constantly shocked by the transcending showcases of tomes; the dubiously roosted books seeming like high jumpers holding back to plunge to the earth beneath. I wind up tipping-toeing around the pyramid tables, holding my breath to shield their drop from occurring. I filter the plenty of racks for something to peruse. At that point, all of a sudden, I see it. Concealing ceaselessly, reclined against a virus metal rack, is the one I need; my book of decision, Ready Player One by Ernest Cline. The reflexive red and yellow book coat remains in sharp complexity to the cruel, dulled earthy colored of its roost, similar to a square apple swinging from a contorted tree. The fresh, coat edges fall like a perfectly creased skirt around a solid tough support. Decorated letters delicately raise themselves to my eyes as though to state, ââ¬Ëhelloââ¬â¢, and offer me to take them home. I spy formally dressed ivory pages sandwiched between the dark authoritative, little holes in the dispersing endeavor to shout out with a quiet, ââ¬Ëopen at me firstââ¬â¢. My brain reels at what may be revealed once I take it home, do I dare? The hardback emanates such a longing to me, that I can't prevent a tenderly shuddering hand from connecting and lifting it off the edge. From the start contact, the novel is cool and smooth underneath warm small fingers. The engraved title on the bookââ¬â¢s sleeve moves underneath my fingertips, as tenderly slanting mountains encompassing wide broad valleys. Following outside the lettering, I discover the remainder of the spread faintly much the same as sandpaper, and step my fingers back. I rest the summary on level palms to feel for its weight length. It isn't light to the point that it might be confused with an insignificant picture book, yet it doesn't convey enough weight as War and Peace would. It would make a beautiful example in my developing gather. I softly run my fingertips across shut pages, relishing the moment detail of befuddled page lengths. Consequently, I soothingly open the story sufficiently only to hear it mumble to me. My ears take pleasure in the abrupt acknowledgment of many little winged animals rippling, as though frightened by somebody gallivanting through their natural surroundings. Shutting the cover on this happiness, I am met by the popping fly of the bookââ¬â¢s spine; a tribute to a thundering fire that would be hanging tight for us once we arrived at home. Murmuring delicately, I advance toward the front of the store to buy my guilty pleasure. I get over the coat just to discover the washing of my hand brings to mind the delicate stew of margarine in a hot container upon the oven. For a moment, my craving for my book is quickly overshadowed by my yearning, as I place my prize upon the cashierââ¬â¢s stand. The reverberating crash seems like a dropped bag on a marble floor in a vacant air terminal, constantly stronger then you anticipate that it should be. I swipe my Mastercard as the grinning youngster behind the register: hastily envelops my fortune by plastic, puts a paper receipt inside the sack, presents me with my buy, and pushes me towards the exit. Exiting, I have a feeling of expectation working inside my chest. I have my prize, and all that remaining parts is to return home to the wellbeing of my calm room and disconnected seat. My breath gets in my throat as I consider how superb it will be to savor the primary composed expressions of the story. I envision myself like Neil Armstrong, aside from stepping into another dream and not onto the moon. The commute home is defaced with unlimited lines of vehicles slowing down at numerous stoplights. We beat between the gas and brake pedals, similar to the jerky movement of a springy pony at an open play area. The consistent shaking forward and back has begun to gradually quiet me to rest, so I turn up the air, out of the blue puffing the pack around my prize. Quickly, the vents drive the aroma of new paper into my face, I inhale profoundly. The waiting zest of matured cowhide and printer ink helps me to remember extended periods of time nestled into the calm, having a great time an authorââ¬â¢s overwhelming language. I gradually breathe out my esteemed lungful of air, when I notice I am close enough for my home. My heart jumps at the memory of my quieted home; its serenity will just add to the mitigating minutes I plan on going through with Mr. Cline, a departure from the hustle of clamor. Maneuvering into my carport I get a twinge in my heart of something turned out badly, similar to the smell of approaching precipitation before a huge tempest. The vehicle entryway hammering ought to be booming, however its clamor is muffled by the crazy pounding of a bass drum. Advancing into the house, the conflict of a high cap cymbal shakes the glass, unmistakably helping me to remember lightning doing likewise during the last tempest. By one way or another, I get the particular inclination that my endeavors to have a tranquil, loosened up quiet perusing time will be bested by the racket nearby. Also, wouldnââ¬â¢t you get it, I was correct.
Thursday, July 30, 2020
Status Check is now Open! - UGA Undergraduate Admissions
Status Check is now Open! - UGA Undergraduate Admissions Status Check is now Open! The UGA status check page is now open and freshman applicants are now able to see our final decisions. Again, I will be putting up three different posts (UVA has done this very well, so I am using their great idea) concerning the three different decisions. I will not have data until early next week on overall applicants, admits, wait-list and denied statistics, but I will post it when I have it. It has been a crazy week, so I was not able to get everything together for the data yet, but be patient and I post it when I can. Go Dawgs!
Friday, May 22, 2020
Human Frailty in Othello Essay - 1544 Words
Human Frailty in Othello Tragedy is an intrinsically human concept; tragic heroes are damned by what they themselves do. Othello is not so much felled by the actions of Iago, but by a quality all people possess-- human frailty. Accordingly, Othello is not a victim of consequences, but an active participant in his downfall. He is not merely a vehicle for the machinations of Iago; he had free agency. Othellos deficiencies are: an insecure grasp of Venetian social values; lack of critical intelligence, self-knowledge, and faith in his wife; and finally, insecurity-- these are the qualities that lead to his own downfall. Othello is the Cultural Other in Venetian society, and while he is very learned, it is probable thatâ⬠¦show more contentâ⬠¦3. 400-401). He lacks the critical intelligence to doubt Iago, because Othello feels his masculinity is damaged by even the mere suggestion that he has been cuckolded. His insecure grasp of social and human values results in placing his faith in Iago, over his supposed beloved. His immature romanticism allows passion to override his critical intelligence (as evidenced by his epileptic fits, triggered by superfluous emotion), and results in blindness to the pitfalls that surround him. In the Anthony Hopkins film version, Othello is foaming at the mouth, and seems to get progressively more insane. By the middle of the play, Othello has already believed his newlywed wife has been unfaithful to him; his only relief must be to loathe her (3. 3. 266-267). He strikes his chest, and it hurts his hand, so hardened is his heart. He believes Iago so fully, that all [his] fond love thus do I blow to heaven/ Tis gone/Arise, black vengeance, from hollow hell; this man quickly converts love into hate, as the em otions do seem to go hand-in-hand (3. 3. 442-443). In Act III, Scene III, Iago pledges himself to Othello forever; Othello is being gradually pulled down to Hell (476). It is this unequivocal acceptance of Iagos slipshod evidence over Desdemonas vehement denials that indicate he is not asShow MoreRelatedOthello By William Shakespeare s Othello1893 Words à |à 8 PagesShakespeareââ¬â¢s tragedy Othello deals with various issues in the tragic vein of tragedies such as Hamlet and King Lear. Marriage and associated issues stands out as a key theme and preoccupation in Othello. In Othello, various issues such as race, patriarchal attitudes and other general human frailties complicate marriages. Shakespeare portrays marriage in a very bleak light. In the beginning of the play, we see that marriage is not only a union of man and his wife but also involves various familialRead MoreTheme Of Feminism In Othello1006 Words à |à 5 Pageslust, or betrayal, Shakespearean themes never cease to deliver a cacophony of chaos to the stage. Shakespeareââ¬â¢s Othello, however, carries a theme that ultimately leads to the demise of its secondary heroin, Emilia. Was Emiliaââ¬â¢s feminism-forward outspokenness against Othelloââ¬â¢s patriarchal society worth her paying the ultimate price? Yes. Emilia is the handmaiden to the wife of Othello, Desdemona, and the wife of the storyââ¬â¢s antagonist, Iago. Emilia frequently spends abundant amounts of time withRead MoreOthello, By William Shakespeare1134 Words à |à 5 PagesIn Shakespeareââ¬â¢s play Othello, there is love, jealousy, death and of course, a tragic hero. A tragic hero is one who experiences an inner struggle due to some flaw within his/her character; that struggle results in the fall of a hero. According to Aristotle, ââ¬Å"A tragic hero is a character who is not eminently good and just, yet whose misfortune is brought about not by vice and depravity, but by some error or frailtyâ⬠¦Ã¢â¬ (Aristotle, Poetics). Othello is an intricate play that dwells into the privateRead MorePortrayal Of Women In Othello Essay1102 Words à |à 5 PagesWilliam Shakespeareââ¬â¢s play, The Tragedy of Othello: The Moor of Venice, is set during the Renaissance period, therefore men treat women as if they are objects. Even though men treat women badly they still stand up for their beliefs. They are obedient when their husbands tell them to do something but they are still direct even if the outcome is negative. The women in Othello are obedient but straightforward with their opinions because Desdemona married someone unsuitable, without her fatherââ¬â¢s approvalRead MoreConflict Essay- English1596 Words à |à 7 Pagesantagonists against nature, contradictory emotions or opposing forces, manipulating an individual to fulfill the desire of oneââ¬â¢s unmeasured flame. Applying a prevailing and emphatic depth into the concept of internal and external conflict, the texts Othello composed by Shakespeare and 1984 devised by George Orwell, attracts the audience to see, feel and experience the compelling turmoilââ¬â¢s that erupt. The consequences of racial discrimination, the contrasting of a democracy and a totalitarian societyRead MoreWilliam Shakespeare s Othello, The Moor Of Venice Essay1251 Words à |à 6 PagesIn the play Othello, the Moor of Venice by William Shakespeare, the women take on various roles in this Venetian society. The roles include committing fraud, playing the part of the victim, and playing the part of the hero. In this Venetian society in the 1600s, the women served major purposes and were vital to keep the towns running. However, the women also faced being victimized and stereotyped in this man-run society. Women were inferior to men, treated unequally, and women were also viewed asRead MoreOthello Notes2305 Words à |à 10 PagesOthello Notes: Assessment Objectives: AO1: A consistently fluent, precise writing, using critical terminology to present a coherent and detailed argument in which the question is well understood and answered. AO2: Well developed, analytical and consistently detailed discussion of effects of language, form and structure and ways in which it affects the audience. AO3: Well informed and detailed discussion of different readings of the text by various audiences, as well as different criticalRead MoreFeminism In Othello Essay957 Words à |à 4 PagesEmilia is often named ââ¬Å"the feminist of Othelloâ⬠by scholars and critics because of her, seemingly, fiery independence among a sea of submissive women ( ââ¬Å"Act Four: The Feminist of Othelloâ⬠17). The characterization of a woman who speaks out for herself suggests that Shakespeare thought progressively; because during that time, the Elizabethan era, women were mere objects rather than human beings. The plot of Othello revolves around the misgivings of poor communication and lack of trust among the charactersRead MoreWhat Is the Genre of Woyzeck?1640 Words à |à 7 PagesBy examining what is meant by the concept of theatrical ââ¬Ëgenreââ¬â¢, how would you characterize the genre of Woyzeck? Today, whenever somebody is asked to think of a typical Tragedy, his or her immediate answer would be; Hamlet, Othello, Macbeth, possibly even King Lear. This is because these plays constitute what is conventionally known as a Tragedy. They mostly follow the conventions outlined in Aristotleââ¬â¢s Poetics, and have characteristics recognisable of a Tragedy, for example, the tragic hero beingRead More Female Characters in William Shakespeares Othello Essay examples2137 Words à |à 9 PagesFemale Characters in William Shakespeares Othello Throughout history, womenââ¬â¢s place and role in society has vastly changed, as well as their sexuality. In the Victorian era, it was considered abnormal for women to masturbate or feel pleasure from sexual intercourse with her husband. They were led to believe that they were sick, and thus went to their doctor who would ââ¬Å"ridâ⬠them of their ââ¬Å"feverâ⬠through vaginal stimulation. The patriarchal society did not want women to know or understand
Sunday, May 10, 2020
The Meaning of College Essay Topics for a Straight White Middle Class Male
The Meaning of College Essay Topics for a Straight White Middle Class Male The Ultimate Strategy for College Essay Topics for a Straight White Middle Class Male Coming from an extremely modest charter middle school, higher school was rather shocking. Students wouldn't be concerned with disappointing teachers or receiving punishments that aren't appropriate. As a way to address racial discrimination, every school in the united states, students and staff, will need to take part in anti-racial discrimination programs, to halt the advancement of racial discrimination. Many possible students may not wish to take part in the lessons provided, for they feel uncomfortable or simply do not care about the problem accessible, but will be made to partake in the lessons. Bear in mind that every paragraph should end with a conclusion that is a quick sentence showing the most important idea of the section. Arguments always happen in a particular context. Each of your argument ought to be in a different paragraph. The New Fuss About College Essay Topics for a Straight White Middle Class Male There are several different causes of discrimination, and among the most frequent causes is parents that always teach their children to believe the way that they do. In all instances, be sure you'll be in a position to work with a specific sense of sincerity. This feeling of uniqueness can be based on several different elements. Think of the methods to remove all their uncertainties and explain it in your words. Some sociologists think that social inequality contributes to crime. Discrimination is a rather serious issue for the contemporary society. Social Inequality Leads To Crime There are several distinct kinds of social inequality. Inequality, ignorance and prejudices cause a great deal of negative results on several levels. Obviously, any sorts of discrimination are threats for this kind of idea. Racism is a crystal clear reality in our society which affects all people. The racism is broken into few types. Though it's often racism proceeds to shatter and destroy lives. Understanding College Essay Topics for a Straight White Middle Class Male If you intend to put up such essays for everybody to read, make sure your words do not lead anyone to follow along with the incorrect path of treatment, medication or exercise. Poch confesses even a little error or two won't necessarily kill your probability of getting inas long as it is not on purpose. Now it's your job to put what you've learnt to good use!! There may be an issue of running out of options in such conditions but attempt to provide the topic a little of your perception also. There's a small danger that she'll find a reader who won't accept the Goth'' culture Carrie describes, but most readers will adore the manner Carrie approaches her topic together with her straight-shooting style. You cannot do both, because it is going to be too confusing for your readers. However, there are some who like to pursue it like a pastime. I really like knowing that everybody is listening to my story. Nevertheless, it's a disturbing part of the general American heritage. I only want to say, even if it's the case that you don't want to speak about it, I'm here whenever you do. I felt it was necessary to sacrifice a single culture so as to partake in another, as a way to fit in. I wished to be part of culture which didn't stink. College Essay Topics for a Straight White Middle Class Male Options The interaction ought to be sustained. It's also important to know different forms of discrimination that exist, in addition to their consequences on the people's lives. Now this problem is actively discussed, since in every nation, there's some form of discrimination, which certain groups of individuals suffer from. Maybe it does not exist where you reside, but any place that there'll be adults, teens, or children of distinct races interact together there's a chance of pr ejudice. If You Read Nothing Else Today, Read This Report on College Essay Topics for a Straight White Middle Class Male Some might discover that it's soporific, whereas others see it like a medium to share their creativity. These sources will aid the writers in discovering they had not planned. You have a complete collection of things to compare. The quantity of research you have to do will vary, depending on the topic. Hence, if you'd like to balance your academic and family pressure side-by-side, you can just find absolutely free essay samples so you may have a simple idea of writing proper essays. Oh, it's so tough to get work. Roberts worries that students have a tendency to be too conservative with essays and are frightened to take risks. If somebody near you has felt discrimination, you might wish to consider college essays writing help on such topic. College Essay Topics for a Straight White Middle Class Male - Is it a Scam? Most individuals won't directly discrim inate different races, but it doesn't indicate it doesn't happen everyday. Together with these new laws, an organization known as the Ku Klux Klan was formed to intimidate and halt the advancement of black individuals in society. Moreover, several types of discrimination have an immediate influence on a specific group, and an extra effect on other groups. At the exact same time, there's also indirect discrimination, which is normally discussed not so wide and actively.
Wednesday, May 6, 2020
A Critique of Linda Prineââ¬â¢s ââ¬ÅAbortion is not a bad thingââ¬Â Free Essays
In her article ââ¬Å"Abortion is not a bad thingâ⬠, published on June 24, 2013, the family medicine practitioner and the founder of the Reproductive Health Access Project medical director Dr. Linda Prinehighly defend and stick to the issue that abortion itself as an experience can be positive however our culture fails to praise the women rights in taking life-term decision and demonizes women instead for having an abortion. She also shed the light on the importance of supporting the women on abortion to destigmatize the associated shame. We will write a custom essay sample on A Critique of Linda Prineââ¬â¢s ââ¬Å"Abortion is not a bad thingâ⬠or any similar topic only for you Order Now Prine sees that it is quite enough to struggle for education and work, and here comes abortion to postpone having childrenuntil better ambience for rising children is ensured. Hearing and feeling that the tough jobs they do in the societies as expertized women are highly recognized, appreciated and respected is much needed. The articleââ¬â¢s writer begins her article with disconcertion by the allegation that women getting abortionsare being to an extent distressed. It is the responsibility of our culture that ruin the image of women for obtaining an abortion while the abortion familiarity itself can be affirmative. The main target is to enlarge the right to have abortions while decreasing the dishonor associated with it, so that the society as whole will recognize it as very mutual and an ordinary part of life. Assertion from physicians, supporting women and their decisions, aims a long way towards removing the shame of abortion. Women are almost encouraged to bring a close person to support them and then to praise that person, for the way in which he strengthens her and their relationship. While activists for abortion rights pretend that lessening the abortions number is mostly important. Prine disagrees and argues that unwanted births is the calamity, it should be decreased. A woman can be a super mother only when she is ready and enough prepared for it, in other words after she had pursued for good education and work. Thatââ¬â¢s why it was good to postpone children and here appears why abortion is not a bad thing. A wise usage of social power gives respect to all women, as well as their decisions, supportive relationships and experience which results in surpassing most of the social obstacles that blocks social development. In the article, the author Linda Prine wants to convince us, using her life and career experience as a woman and as a family medicine practitioner. She had succeeded to an extent in doing her aim. She was absolutely credible in trying to persuade us that abortion is really not a bad thing bytaking advantages of the readers emotions using a sensual languageby giving several life examples she used to experience in her career that made her certain that abortion is not that bad as the 16-year-old couple who came scared to her clinic, and how they relaxed a bit after she talked to them when they realized that she is helping them as well as the two mother/daughter pairs who realized after her visit how great their jobs when they are supporting their daughters goals in getting more education. The audience definitely empathizes with the womenââ¬â¢s psychological state and the fear she feels before abortion and that after, this is the emotional appeal, it is an appeal to pathos. Being the founder of the Reproductive Health Access Project medical director and a writer, people feels that the issue is more granted as an idea to adopt, and this is was another reason why she was credible. Here appealing to ethos is absolutely achieved as this is a clear zoom into the writerââ¬â¢s character and his respect to the reader. She insists that our societies are responsible for damaging the womanââ¬â¢s image for having an abortion which can be almost positive, while they should be supportive and respective for the women and their decisions, supportive relationships and experience. The logical reasoning she followed is well detailed and with no hard terminology it could be followed easily. She emphasizes the necessity to decrease the shame associated with abortion so that it becomes something normal and undebatable. She suggests and encourages as a doctor the support of women having abortion especially from the closely people. She also sees abortion as positive in postponing children till better life situations are achieved. Logical fallacies were evaded which is excellent. To make it simple, she makes the reader well understand the importance and how serious the issue is especially when she related the positive effects of abortion to the women world especially and to the society as a whole. She is absolutely a good persuader. One cannot deny that Prine was so logical in the way she presented her issue and supported it. Not only she let the issue enter into the readerââ¬â¢s heart, but she made that heart think how tender she is to be concerned with the promotion of the women rights by this way. She was biased in developing her argument as she neglected to state any counterargument. She even disagreed with the activists of abortion rights that they need to decrease the number of abortions. She insists instead that abortion is very normal. Outlining few opposing views would have made her argument better, no counter arguments were present. As a family doctor and writer at the same time, her role should notbe limited only to ease the abortion for females, her duty must extend to awake young couples before committing the mistake of unwanted pregnancy. In her argument she only stuck to the necessity of social acceptance of women having abortions but she didnââ¬â¢t mention any moral, religious and conscious overview. Abortion is against God, it is denied by all religions. Abortion is also related to the basic human values, so women should be less egoistic and more humanitarian regarding those unborn babies. Abortion is not safe, she shouldnââ¬â¢t have muted this issue as a family doctor as she should know that abortion industry is not regulated and it can be accompanied with a mother death. She should have stated medical and social wise that it is a better alternative than having a defective fetus or child. All of those were not mentioned. Prineââ¬â¢s work presented abortion as a solid basis towhistle blow the need of the women rights in the society and the importance of respecting them. An underestimation of the effects of mentioning the counterarguments decreased her creativity. Although the way she presents her claim is so logical. How to cite A Critique of Linda Prineââ¬â¢s ââ¬Å"Abortion is not a bad thingâ⬠, Papers
Wednesday, April 29, 2020
Roe Vs. Wade The Decision And Its Impact On American Society Essays
Roe vs. Wade: The Decision and its Impact on American Society "The Court today is correct in holding that the right asserted by Jane Roe is embraced within the personal liberty protected by the Due Process Clause of the Fourteenth Amendment. It is evident that the Texas abortion statute infringes that right directly. Indeed, it is difficult to imagine a more complete abridgment of a constitutional freedom than that worked by the inflexible criminal statute now in force in Texas. The question then becomes whether the state interests advanced to justify this abridgment can survive the ?particularly careful scrutiny' that the Fourteenth Amendment here requires. The asserted state interests are protection of the health and safety of the pregnant woman, and protection of the potential future human life within her. But such legislation is not before us, and I think the Court today has thoroughly demonstrated that these state interests cannot constitutionally support the broad abridgment of personal liberty worked by the existing Texas law. Accordingl y, I join the Court's opinion holding that that law is invalid under the Due Process Clause of the Fourteenth Amendment" (Craig and O'Brien 17). On January twenty-second, 1973 Justice Harry Blackmun delivered the opinion of the Supreme Court regarding the Roe vs. Wade case. A pregnant single woman, "Jane Roe," brought a class action lawsuit challenging the constitutionality of the Texas criminal abortion laws, which proscribed procuring or attempting an abortion except on medical advice for the purpose of saving the mother's life. Norma McCorvey, the real name of the plaintiff, was young and divorced at the time, searching for a solution to her unplanned pregnancy. "No legitimate doctor in Texas would touch me," stated McCorvey. "There I was ? pregnant, unmarried, unemployed, alone and stuck" (Craig and O'Brien 5). The plaintiff's assertion was that prohibiting abortion at any time before birth violated a woman's constitutional right to privacy. The Supreme Court later agreed with Mrs. McCorvey, justifying the legality of abortion under the fourteenth amendment. A person's right to privacy now extended to choosing an abortion . Although the Court avoided the issue of when life actually begins, abortion became legal under this landmark Supreme Court decision. The debate over the legality of abortion had taken place in America for several decades, and the final decision rendered by Roe vs. Wade resonated among all Americans, influencing society to date. Until the last third of the nineteenth century, when it was criminalized state by state across the land, abortion was legal before "quickening," which is approximately the fourth month of pregnancy. Colonial home medical guides gave recipes for instigating abortions with herbs that could be grown in one's garden or easily found in the woods. By the mid-eighteenth century, commercial preparations were widely available. Unfortunately, these drugs were often fatal. The first statutes regulating abortion, passed in the 1820s and 1830s, were actually poison-control laws: the sale of commercial abortifacients was banned, but abortion itself was not outlawed. Despite these new laws, the abortion business was booming by the 1840's, including the sale of illegal drugs, which were widely advertised in the popular press. However, this trend would soon change. Following the 1840's, abortion would soon be under attack, and a string of anti-abortion laws would be passed until the twentieth century. The leading force behind the criminalization of abortion was physicians and the American Medical Association. The AMA was founded in 1847, and the illegalization of abortion was one of its highest priorities. To the growing movement, "abortion was both an immoral act and a medically dangerous one, given the incompetence of many of the practitioners then" (Joffe 28). However, the opposition went beyond these factors. To many people during the end of the nineteenth century, abortion represented a threat to male authority and the traditional role of a woman in the time period. Abortion was a symbol of unbridled female sexuality, expressing selfish and self-indulgent qualities. The AMA's Committee on Criminal Abortion expressed this view blatantly in 1871. "She yields to the pleasures ? but shrinks from the pains and responsibilities of maternity; and, destitute of all delicacy and refinement, resigns herself, body and soul, into the hands of unscrupulous and wicked men" (Joffe 29). As the twentieth
Friday, March 20, 2020
Veterans Disability I Disability Attorneys of Michigan
Veterans Disability I Disability Attorneys of Michigan Remembering 9/11 As we begin our Monday morning, it is important to take a moment to remember this historic day that changed so many American lives. Today we remember all of the victims and families of victims from the September 11thà attacks.Today there will be 9/11 Memorial services around the country to pay tribute to those we lost.At the Disability Attorneys of Michigan, we would like to pay our respects to the victims and families of victims of the 9/11 attacks sixteen years ago. And we would like to thank our incredible military for working so hard to keep our country safe.We will never forget.Disability Attorneys of Michigan. Compassionate Excellence. DA Michigan, Disability Attorneys, Disability Attorneys of Michigan, Veterans Disability, Veterans Disability Attorneys, Veterans Disability lawyer, Veterans Disability Lawyers
Wednesday, March 4, 2020
An Overview of the USS Massachusetts
An Overview of the USS Massachusetts In 1936, as the design of the North Carolina-class was being finalized, the US Navys General Board met to converse regarding the two battleships that were to be funded in Fiscal Year 1938.à Though the Board preferred building two additional North Carolinas, Chief of Naval Operations Admiral William H. Standley opted to pursue a new design.à As a result, construction of these battleships was delayed to FY1939 as naval architects commenced work in March 1937.à While the first two ships were officially ordered on April 4, 1938, the second pair of vessels was added two months later under theà Deficiency Authorization which passed due to rising international tensions.à Though the escalator clause of the Second London Naval Treaty had been invoked allowing the new design to mount 16 guns, Congress required that the battleships stay within the 35,000-ton limit set by the earlier Washington Naval Treaty. In designing the new South Dakota-class, naval architects created a wide array of plans for consideration.à A principal challenge proved to be finding ways to improve upon the North Carolina-class while staying within the tonnage limit.à The answer was the design of a shorter, by approximately 50 feet, battleship that incorporated an inclined armor system.à This offered better underwater protection than earlier vessels.à As naval leaders called for vessels capable of 27 knots, designers sought a way to obtain this despite the reduced hull length.à This was achieved through the creative layout of machinery, boilers, and turbines.à For armament, the South Dakotas equaled the North Carolinas in mounting nine Mark 6 16 guns in three triple turrets with a secondary battery of twenty dual-purpose 5 guns.à These weapons were supplemented by an extensive and constantly changing complement of anti-aircraft guns.à Assigned to Bethlehem Steels Fore River Shipyard, the third ship of the class, USS Massachusetts (BB-59), was laid down on July 20, 1939.à Construction on the battleship advanced and it entered the water on September 23, 1941, with Frances Adams, wife of former Secretary of the Navy Charles Francis Adams III, serving as sponsor.à As work moved towards completion, the US entered World War II after the Japanese attack on Pearl Harbor on December 7, 1941.à Commissioned on May 12, 1942, Massachusetts joined the fleet with Captain Francis E.M. Whiting in command.à Atlantic Operations Conducting shakedown operations and training during the summer of 1942, Massachusetts departed American waters that fall to join Rear Admiral Henry K. Hewitts forces which were gathering for the Operation Torch landings in North Africa.à Arriving off the Moroccan coast, the battleship, heavy cruisers USS Tuscaloosa and USS Wichita, and four destroyers took part in the Naval Battle of Casablanca on November 8.à In the course of the fighting, Massachusetts engaged Vichy French shore batteries as well as the incomplete battleship Jean Bart.à Pounding targets with its 16 guns, the battleship disabled its French counterpart as well as struck enemy destroyers and a light cruiser.à In return, it sustained two hits from shore fire but received only minor damage.à Four days after the battle, Massachusetts departed for the US to prepare for redeployment to the Pacific. To the Pacific Transiting the Panama Canal, Massachusetts arrived at Noumà ©a, New Caledonia on March 4, 1943.à Operating in the Solomon Islands through the summer, the battleship supported Allied operations ashore and protected convoy lanes from Japanese forces.à In November, Massachusetts screened American carriers as they mounted raids in the Gilbert Islands in support of the landings on Tarawa and Makin.à After attacking Nauru on December 8, it aided in the assault on Kwajalein the following month.à After supporting the landings on February 1, Massachusetts joined what would become Rear Admiral Marc A. Mitschers Fast Carrier Task Force for raids against the Japanese base at Truk.à On February 21-22, the battleship helped defend the carriers from Japanese aircraft as the carriers attacked targets in the Marianas. Shifting south in April, Massachusetts covered the Allied landings at Hollandia, New Guinea before screening another strike against Truk.à After shelling Ponape on May 1, the battleship departed the South Pacific for an overhaul at Puget Sound Naval Shipyard.à This work was completed later that summer and Massachusetts rejoined the fleet in August.à Departing the Marshall Islands in early October, it screened American carriers during raids against Okinawa and Formosa before moving to cover General Douglas MacArthurs landings on Leyte in the Philippines.à Continuing to protect Mitschers carriers during the resulting Battle of Leyte Gulf, Massachusetts also served in Task Force 34 which was detached at one point to aid American forces off Samar. Final Campaigns Following a brief respite at Ulithi, Massachusetts and the carriers returned to action on December 14 when raids were mounted against Manila.à Four days later, the battleship and its consorts were forced to weather Typhoon Cobra.à The storm saw Massachusetts lose two of its float planes as well as one sailor injured.à Beginning on December 30, attacks were made on Formosa before the carriers shifted their attention to supporting Allied landings in Lingayen Gulf on Luzon.à As January progressed, Massachusetts protected the carriers as they struck French Indochina, Hong Kong, Formosa, and Okinawa.à Beginning on February 10, it shifted north to cover raids against mainland Japan and in support of the invasion of Iwo Jima. à à à à à In late March, Massachusetts arrived off Okinawa and commenced bombarding targets in preparation for landings on April 1.à Remaining in the area through April, it covered the carriers while fighting off intense Japanese air attacks.à After a short period away, ââ¬â¹Massachusetts returned to Okinawa in June and survived a second typhoon.à Raiding north with the carriers a month later, the battleship conducted several shore bombardments of the Japanese mainland beginning on July 14 with attacks against Kamaishi.à Continuing these operations, Massachusetts was in Japanese waters when hostilities ended on August 15.à Ordered to Puget Sound for an overhaul, the battleship departed on September 1. Later Careerà Leaving the yard on January 28, 1946, Massachusetts briefly operated along the West Coast until receiving orders for Hampton Roads.à Passing through the Panama Canal, the battleship arrived in the Chesapeake Bay on April 22.à Decommissioned on March 27, 1947, Massachusetts moved into the Atlantic Reserve Fleet.à It remained in this status until June 8, 1965, when it was transferred to the Massachusetts Memorial Committee for use as a museum ship.à Taken to Fall River, MA, Massachusetts continues to be operated as a museum and memorial to the states World War II veterans.à à Selected Sources: DANFS: USS Massachusetts (BB-59)NHHC: USS Massachusetts (BB-59)Battleship Cove Museum
Sunday, February 16, 2020
Evolution of supply chain management Assignment
Evolution of supply chain management - Assignment Example b) Integration era ââ¬â the integration era marked the use of Electronic Data Interchange (EDI) systems and Enterprise Resource Planning (ERP) systems. This era stressed on value added and cost reduction supply chain management through integration. c) Globalization era ââ¬â the globalization era was characterized by the emergence of global system of vendors and suppliers and the expansion of supply chain over national and international boundaries. In this era, organizations aimed to attain competitive advantage by creating value added services and products. At the same time, it aimed to reduce costs through global sourcing. d) Specialization era (Phase I ââ¬â Outsourced manufacturing and distribution) ââ¬â this era saw the creation and development of the specialization model which composed of numerous individual supply chains specifically for products, suppliers and customers who worked together to manufacture, design, market, distribute, sell and service a particular product. e) Specialization era (Phase I ââ¬â Supply chain management as a service) ââ¬â the commencement of warehouse management, transport brokers and freight carriers has matured into the aspects of collaboration, supply planning, performance management and execution. All this was possible due to the breakthroughs achieved in technology. f) Supply Chain Management 2.0 ââ¬â this terminology was coined to explain the changes within the supply chain industry and the evolution of methods, tools and processes used to manage resources now. It is very important for an organization to develop supply chain strategy as it creates value for the organization. The first step is to develop a supply chain strategy. For this the first step is to understand the business strategy. If the business strategy is to reduce costs, then the supply chain strategy must be developed to support the same. It is important to find out supply chain competencies and leverage them for the benefit of th e organization. The next step is to access the extended supply chain. This is attained by conducting a realistic, detailed monitoring of capabilities existing within the firm and the extended supply chain. Once the assessment is complete it is very necessary to prioritize and review the recommendations, define the risks, validate the opportunities and the requirements for implementing the supply chain strategy. 2. Explore strategies used by organizations to develop and maintain effective supplier relationships: ? determine suitable strategies with regard to the development of relationships with suppliers, using appropriate web-based technologies ? evaluate the effectiveness of strategies used by an organization ? devise effective systems for relationship maintenance The breakthroughs achieved in web-based technology have made us realize the importance of information exchange that can play a vital part in the competitive strategies adopted by different organizations. It is possible t o maintain good business to business and business to consumer supplier relationships by means of web based techniques. Extensive telecommunication networks are used to develop and maintain effective supplier relationships. Electronic trading is one such method which has effectively helped to maintain vendor relationships (Croom 2000). Another web based technique used to maintain effective supplier relationship is that of electronic warehouse management. It is now possible to inform a client well in
Monday, February 3, 2020
Discuss posthumanism and the fears concerning it that are addressed in Essay
Discuss posthumanism and the fears concerning it that are addressed in pop culture - Essay Example The world of pop culture has had a particular influence on art from the early 1960s on, through Pop Art. John Storey in his book "Cultural Theory and Popular Culture" defines popular culture as a culture that is related to masses and can be defined as an "authentic" culture of the people. It is commonly seen as a commercial culture that is mass produced for mass consumption Items of popular culture most typically appeal to a broad spectrum of the public. So a culture that is related to masses consequently represents the common ideas, practices, theories, customs, traditions and values of the people in all aspects of life, or in other words, it represents all that is truly human. As compared to the theories of Pop culture, Post humanism, as the name of a discourse, suggests an episteme which comes "after" humanism ("post-humanism") or even after the human itself ("post-human-ism").
Saturday, January 25, 2020
Genetic Influences on Salmonella Formation
Genetic Influences on Salmonella Formation IHF Gene Influences Salmonella Enteritidis Biofilm Formation Integration Host Factor (IHF) is important for biofilm formation by Salmonella enterica Enteritidis Bruna Leite, Catierine Hirsch Werle, Camila Pinheiro do Carmo, Diego Borin Nbrega, Guilherme Paier Milanez, Cristina E. Alvarez-Martinez, Marcelo Brocchi Abstract Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments where it infects poultry and eggs. Once established, biofilms are difficult to eradicate, due to their high resistance compared to planktonic cells, causing serious problems in industry and public health. In this study, we evaluated biofilm formation in wild-type strains of S. enterica Enteritidis and in ihf mutants employing different microbiology techniques. Our data indicate that ihf mutants display impaired biofilm formation, with a reduced of matrix formation and a decrease in CFU and metabolic activity. Phenotypic analysis indicated a deficiency in curli fimbriae expression and in cellulose production and pellicle formation. These results show that IHF has a regulatory role in biofilm formation in S. enterica Enteritidis. Keywords: Biofilm, Salmonella enterica Enteritidis, Polysaccharide matrix, Curli fimbriae, Cellulose, Integration Host Factor. Introduction A biofilm is defined as a bacterial colony adherent to a solid surface, which secretes a protective exopolysaccharide matrix. Every natural wet surface is a potential substrate for microbial biofilms. These sessile multicellular microbial consortia are embedded within self-produced extracellular polymeric substances (EPS). In food handling facilities, biofilms can be particularly problematic The ability to form biofilms is also an important factor in the virulence of S. Enterica. S. enterica subspecies I serovar Enteritidis is a leading cause of salmonellosis worldwide, and has emerged as one of the most important foodborne pathogens for humans. It is mainly associated with consumption of contaminated meat and eggs of poultry. A number of studies have demonstrated that S. enterica is capable of forming biofilms on a wide variety of contact surfaces, and the formation of biofilms may improve the ability of these organisms to resist stresses such as desiccation, extreme temperatures, antibiotics, and antiseptics. Biofilm formation allows S. enterica to survive for long periods in a poultry farm environment and to contaminate poultry meat and eggs, which remain the leading vehicles of salmonellosis outbreaks Many factors are involved in biofilm development. Curli fimbriae and cellulose are the major components of biofilm formed by S. enterica, whereas capsular polysaccharide, other polysaccharide-rich compounds such as lipopolyssaccharide (LPS), and a large secreted protein, BapA, also contribute to biofilm formation. Several regulatory genes involved in biofilm formation have been identified The expression of curli fimbriae and cellulose can be assayed phenotypically by growing enteric bacteria on Congo red indicator plates Bacteria may live in planktonic form in liquid media or as biofilms on biotic or abiotic surfaces. They need to adjust their genetic programs in order to switch from one lifestyle to another. The production of bacterial products and behaviours associated with environmental adaptation must be tightly coordinated to optimize the energy consumption. In bacteria, gene expression regulation is exerted primarily at the level of transcription initiation using a large array of transcription factors whose concentrations and activities change depending on specific environmental or metabolic signals. Topological changes in DNA also influence promoter recognition, open complex formation, and gene expression Nucleoid-associated proteins (NAPs) are global regulators of gene expression in bacteria. They alter the topology of DNA by bending, bridging, or wrapping it, leading to DNA transactions and multiple cellular effects that culminate in the modulation of gene expression. Integration-host factor (IHF) is a dimeric NAP that binds DNA in a sequence-specific manner and introduces curvatures of up to 180à °, which influence many aspects of bacterial physiology, including global gene expression, DNA topology, site-specific recombination, and DNA replication. In E. coli and S. enterica Typhimurium, the two IHF subunits-IHFà ± and IHFà ²-can assemble as hetero- or homo-dimers. There is also evidence indicating that the different dimeric forms of IHF regulate different but overlapping sets of genes Based on the global regulatory role of IHF, we hypothesized that this NAP can influence or directly regulate genes involved in biofilm formation in S. enterica Enteritidis. This hypothesis is supported by previous observations demonstrating that IHF activates curli production in S. enterica Typhimurium. Therefore, in this study, we evaluated the role of IHF genes in the initial stages of biofilm formation in S. Enteritidis. To this end, we performed phenotypic studies using isogenic deletion mutants of individual ihf genes (ihfA or ihfB) and a double mutant strain with deletions in both IHF subunits (ihfAB double mutant). Materials and methods Bacterial strains In this study, the S. enterica Enteritidis wild-type strain PT4SEn (IOC4647) provided a by the Fundaà §Ã £o Oswaldo Cruz (FIOCRUZ, Rio de Janeiro, Brazil) was used. The draft genome of this strain was recently published (Milanez et al. 2016). It was found to be pathogenic in a mouse model assay (Carmo et al., unpublished results). The mutants of S. Enteritidis PT4SEn were previously constructed (Carmo et al., unpublished results) by deletion of ihf genes using the lambda Red system by transduction with P22HT phages. Mutant strains were designated as S. enterica Enteritidis PT4SEn ÃâihfA, PT4SEn ÃâihfB, and PT4SEn ÃâihfAB. Bacterial growth conditions and storage Bacteria were cultivated in Luria-Bertani broth (LB) and on Luria-Bertani agar (LBA) plates prepared according to the method of Sambrook and Russell. All strains were stored at -80à °C in 30% glycerol All strains were inoculated from fresh LBA plates into 15 mL LB and grown for 18 à ± 2 h at 37à °C in an orbital shaker at 140 rpm. Cells were harvested by centrifugation (for 5 min at 9,500 g and 4à °C) and resuspended in NaCl (0.9%) adjusted to 0.5 McFarland scale equivalent to 1.5 108 cells/mL prior to use in subsequent assays. Complementation of S. enterica Enteritidis ÃâihfA and ÃâihfB mutants Sequences corresponding to the ihfA and ihfB genes and their regulatory regions were obtained by PCR from the PT4SEn genome using the primers listed in Table 1. The DNA fragments were cloned in the pACYC184 vector (New England Biolabs, USA) between the NcoI and EcoRI restriction sites (restriction enzyme sites in the DNA fragments were introduced via the primers) and the vector was subsequently electroporated into the respective S. enterica Enteritidis mutant strains. Cloning, PCR amplification, electroporation, plasmid extraction, and agarose gel electrophoresis were performed as suggested by Sambrook and Russell (2001). After DNA purification using the Wizardà ® Genomic DNA Purification Kit (Promega Corporation, Madison, USA), Sanger sequencing was performed using 3730XL Applied Biosystems (Foster City, California, USA) by the High Performance Technologies Central Laboratory in Life Sciences (LACTAD, University of Campinas UNICAMP, Campinas, Brazil). Biofilm formation on polystyrene plates Biofilms were formed in 96-well plates (Cell Culture Plate, Nest, Biotechnology Co, China) containing 200 à ¼L of cell suspension (1 106 cells/mL) of S. enterica Enteritidis PT4SEn wild-type or mutant strains in LB supplemented with 0.25% of glucose. Plates were incubated at 37à °C with orbital shaking at 140 rpm for 48, 72, and 120 h. At the end of the incubation period, planktonic cells were carefully removed, and biofilms were washed twice with 200 à ¼L of saline solution (0.9% NaCl). The crystal violet staining method was used to assess total biofilm biomass. Each well of the biofilm plates was incubated with 200 à ¼L of methanol for 15 minutes. Subsequently, methanol was removed and 1% (v/v) crystal violet solution was added, followed by a 5-min incubation period. Wells were washed with distilled water and finally 33% (v/v) acetic acid was added. The absorbance was measured at 570 nm. The colorimetric method based on the reduction of XTT (2,3- bis(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)carbonyl-2H tetrazolium hydroxide; Sigma-Aldrich, USA) was used to determine cell activity (XTT is converted to a coloured formazan salt in the presence of metabolic activity). To each well of the biofilm plate, 200 à ¼L of a solution containing 200 mg/L of XTT and 20 mg/L of phenazinemethosulphate (PMS; Sigma-Aldrich, Ukraine) was added. Microtiter plates were incubated for 3 h at 37à °C in the dark. The absorbance was measured at 490 nm. To assess the number of viable cells in biofilms, 200 à ¼L of saline solution was added to each well before removal of the biofilm by scraping. For each sample, an aliquot of 1 mL (5 wells) was sonicated (20 s with 22% of amplitude; Ultrasonic Processor, Cole-Parmer, Illinois, USA) to promote biofilm disruption. The number of colony forming units (CFU) in biofilms was determined by performing 10-fold serial dilutions in saline solution, plating on LBA plates in triplicate, and incubating for 24 h. Scanning electron microscopy (SEM) of biofilm cells Biofilms of S. enterica Enteritidis PT4SEn wild-type and mutant strains formed in 24-well plates (Well Cell Culture Cluster, Costar) were dehydrated by a 15-min immersion in increasing ethanol concentrations (70, 95, and 100% ethanol [v/v]) and placed in sealed desiccators. The samples were mounted on aluminium stubs with carbon tape, sputter-coated with gold, and analysed with a JEOL JSM-5800LV scanning microscope. All experiments were carried out in duplicate. Biofilm formation at the air-liquid interface Biofilm formation at the air-liquid interface was assessed in S. enterica Enteritidis PT4SEn strains by inoculation of LB cultures without NaCl, followed by incubation at 28à °C without shaking. Every day for 10 days, each isolate was visually examined for pellicle formation. Experiments were performed in triplicate. Expression of curli fimbriae Bacterial colony morphology of S. enterica Enteritidis PT4SEn wild-type and mutant strains was analysed on LB agar without NaCl, supplemented with Congo red (1.01340.0025, Sigma-Aldrich, Germany; 40 à ¼g/mL) and Coomassie brilliant blue G (B0770-5G, Sigma-Aldrich, China; 20 à ¼g/mL). Bacterial cultures were spread on agar plates and the colour and degree of colony rugosity were determined after 96 h of growth at 28à °C. Images were captured with a camera (Nikon P500) and under an HBO 100 Carl Zeiss Illuminating microscope system. Cellulose production The fluorescence exhibited by bacteria after growth of S. enterica Enteritidis PT4SEn wild-type and mutant strains in LB plates with Calcofluor (Fluorescent Brightener 28; F3543-1G, Sigma-Aldrich, China; 200 à ¼g/mL) served as an indicator of cellulose production. Fluorescence was analysed visually using an UV light (366 nm) after 48 h of growth at 37à °C. Statistical analysis Data were analysed using STATA software, version 13.0 (Stata Corp, College Station, TX, USA). Data from all assays were compared using one-way analysis of variance (ANOVA). Sidaks adjustment for multiple comparisons was performed after a significant fitting. The significance level was set at 5%. Results ihf mutants display reduced viability, biomass, and metabolic activity A decrease of about 1-2 log10 in number of viable cells was observed for the ihf mutants in comparison with the wild-type S. enterica Enteritidis PT4SEn strain by CFU counting (Figure 1-A). The differences observed were statistically significant (P < 0.05) for all periods of time evaluated. The introduction of the pACYC184 plasmid carrying ihfA or ihfB was generally associated with an increase in CFUs, but complementation did not completely restore the values to those obtained with the wild-type strain. No statistical differences were observed at 48 and 72 h of incubation between ÃâihfAc and the wild-type strain. The same observation is valid for ÃâihfB after 120 h of incubation (Figure 1-A). These results show that the restoration of ihfA or ihfB gene copies in mutant strains is generally associated with an increase in CFUs in biofilms. The total biofilm biomass, assessed by CV staining of S. enterica Enteritidis PT4SEn and mutant strains is presented in Figure 1-B. An increase in biomass is observed for the wild-type strain over time. However, this effect was not observed for the correspondent PT4SEn ihfAB double mutant. None of the mutants presented an increase in biofilm matrix density at 48 and 72 h of incubation (P < 0.05). The complemented PT4SEn ihfA and ihfB mutants (ihfAc and ihfBc) showed an increase in total biofilm biomass in comparison to the non-complemented mutants (Figure 1-B). All mutant strains exhibited a significant reduction in metabolic activity measured by the XTT assay for cells in biofilm (P < 0.05). In addition, the double mutant (ihfAB) showed the greatest reduction in metabolic activity at 72 and 120 h (Figure 1-C). ihf genes are essential for biofilm structure To further characterize biofilm formation and structure in strains lacking ihf genes, we performed scanning electron microscopy (SEM) analysis of cells in biofilms. As shown in Figure 2, the absence of ihfA or ihfB drastically affects biofilm formation, as null mutants of S. enterica Enteritidis PT4SEn (Figure 2-D, E and F) exhibited a low amount of matrix and small number of cells compared to the wild-type (Figure 2-A). Complementation of ihf gene deletions by a wild-type copy of the corresponding gene promoted a significant restoration of biofilm formation (Figure 2-B and C). Pellicle formation at the air-liquid interface To further characterize the mutant strains with respect to their ability to form biofilms we analysed the biofilm formation at the air-liquid interface of cultures of the different strains. Cultures of the wild-type strain formed a thick and rigid pellicle after 10 days of growth (Figure 3-A). On the other hand, PT4SEn ihfA or PT4SEn ihfB mutant strains formed a less compact and fragile pellicle (not shown). Interestingly, the double mutant strain PT4SEn ihfAB did not form a visible pellicle at all at the air-liquid interface. Instead, cell deposition was observed at the bottom of the tube (Figure 3-B). Complementation with the wild-type copy of ihfA and ihfB restored the phenotype of the single mutants (PT4SEn ÃâihfAc and PT4SEn ÃâihfBc strains), which now formed a thick and rigid pellicle (not shown). Curli and cellulose Since curli and cellulose are important components in biofilm formation, we evaluated the role of IHF on their production. To this end, colony morphology was analysed on LBA plates supplemented with Congo red and Coomassie brilliant blue, as previously described.. enterica Enteritidis PT4SEn wild-type and PT4SEn ÃâihfA and ÃâihfB complemented strains exhibited a phenotype consistent with curli fimbriae and cellulose production, with red, dry, and rough (rdar) colony morphology (Figure 4-A to D). However, the PT4SEn ÃâihfA, PT4SEn ÃâihfB, and PT4SEn ÃâihfAB mutants of S. enterica Enteritidis did not display the same colour and roughness, but instead exhibited a similar, but not identical, smooth and white (saw) morphotype, indicating a deficiency in the expression of curli fimbriae and probably also of cellulose (Figure 4-E to H). The expression of cellulose was also tested by screening the colonies for Calcofluor binding Cellulose production was observed for all strain s evaluated by this method, except for the double mutant ihfAB that was not fluorescent under an UV light source and was considered a poor producer of cellulose (Figure 5). Discussion The presence of microorganisms on food contact surfaces is one of the most common causes of food spoilage and transmission of foodborne diseases. Inadequate cleaning and disinfection of food-processing environments is the cause of major economic losses and represents a serious danger to public health. The ability of microorganisms to adhere and form biofilms makes disinfection even more difficult and challenging Infections with Salmonella enterica Enteritidis represent a major health problem and a significant burden on the food industry. About 80% of the infections are caused by biofilm formation In the matrix of a biofilm, bacteria grow on either biotic or abiotic surfaces, attaching to the surface and to each other, conferring resistance to immunity responses as well as antimicrobial agents As a consequence, antimicrobial treatments typically fail to eradicate biofilms. The need to create effective therapies to counteract biofilm infections is a pressing challenge in the food indus try The growing interest in understanding the regulatory network of gene activities during the transition from a planktonic to a sessile cellular lifestyle, prompted us to investigate the role of IHF in S. enterica Enteritidis biofilm formation. IHF has an important role in the regulation of gene expression and environment adaptability of S. Enterica Therefore, S. Enteritidis deletion mutants for ihfA, ihfB, or both genes (ihfAB) were employed in different assays to analyse biofilm formation. The logic behind this approach is based on the fact that IHF can act as a homodimer (IHFà ±Ã ± or IHFà ²Ã ²) or as a heterodimer (IHFà ±Ã ²) The results presented here indicate an important role of this NAP in the formation of biofilms in S. enterica Enteritidis. All typical biofilm characteristics analysed in this study (CFU, biomass, and cellular metabolic activity) were significantly decreased in S. enterica Enteritidis mutant strains for ihfA, ihfB, or ihfAihfB. The biofilms formed by mutant strains exhibited a decreased matrix density compared with the wild-type strain. Therefore, these results indicate that IHF can influence the initial stage of biofilm formation by S. enterica Enteritidis, as the matrix is necessary in this phase. This is also supported by CV staining and SEM. The colony morphotypes observed in Congo red among wild-type and complemented strains exhibited the rdar morphotype, an indication of curli and cellulose production, while the mutant strains exhibited a similar but not identical saw morphotype, suggesting an altered expression of curli and probably also of cellulose. In fact, bacterial growth in calcofluor-containing medium indicated that the single ihf-mutants were able to produce cellulose, but the ihf-double mutant exhibited some deficiency in the production of this polysaccharide. Previously, Gerstel, Park, and Rà ¶mling demonstrated that the ÃâihfAB double mutant of two S. enterica Typhimurium strains caused a reduction in CsgD expression and an altered rdar morphotype suggesting a role for IHF in curli expression in S. enterica Typhimurium. Curli is expressed by two divergent operons, csgBAC and csgDEFG. CsgD is a major regulator of curli expression and biofilm formation. This gene activates transcription of csgA and csgB that encodes the major (CsgA) and the minor (CsgB) curli subunits In addition, csgD also regulates cellulose production Therefore, IHF plays an important role in biofilm formation in S. enterica Typhimurium. Our results demonstrate a similar role for IHF in the biofilm formation of S. enterica Enteritidis. Despite high genetic similarity, the Enteritidis and Typhimurium serovars differ in various ecological and host-relationship parameters However, the regulation of biofilm formation by IHF in both serovars suggests that IHF plays a cen tral role in S. enterica biofilm biogenesis. However, additional studies of IHF function on biofilm biogenesis in other S. enterica serovars are needed to further clarify this question. In addition, the single ihf mutants also exhibited a phenotypic alteration in biofilm formation, indicating that both subunits are necessary for appropriate biofilm production. In our results, all the ihf mutants showed a deficiency for curli fimbriae production by phenotypic tests. To some extent, a deficiency in cellulose production was also observed, particularly in the double ihf-mutant. The complementation of the ihfA and ihfB mutants by the introduction of a pACYC184 plasmid carrying the wild-type genes reverted the deficiency in biofilm biomass, cell metabolism, and CFUs, but in the majority of the tests the values did not reach those observed for the wild-type strain. This is probably due to a dose effect of IHFà ± or IHFà ², despite the low copy number (about 15 copies per cell) of the plasmid used. In fact, the expression of ihf genes is finely regulated and depends on the growth phase The two operons bcsABZC and bcsEFG are responsible for cellulose biosynthesis in both S. enterica Enteritidis and S. enterica Typhimurium. This was demonstrated by the construction of non-polar mutants of bcsC and bcsE genes that formed a fragile pellicle at the air-liquid interface of LB medium The same authors also showed that cellulose-deficient mutants were more sensitive to chlorine treatments, indicating that the deficiency in the production of extracellular matrix can leave the cells more susceptible to the action of some chemical agents. In our study, IHF mutant strains formed a less compact pellicle in LB compared to wild-type strains. In addition, the ihf double mutant did not form an air pellicle at all, suggesting a role for IHF in the expression of cellulose. These findings corroborate a previous study in which S. enterica Typhimurium ihfAB mutants exhibited reduced bcsC transcription when evaluated by microarray analysis, but further studies are needed to better charact erize the underlying molecular mechanisms. Karaca, N Akcelik, and M Akcelik (2013) also evaluated pellicle formation at the air-liquid interface of 31 S. enterica isolates. They showed that the growth rate of isolates with a rigid pellicle was greater than that of the ones forming a fragile pellicle. Biofilm production at the air-liquid interface can facilitate and contribute to gas exchange, while enabling the acquisition of nutrients and water from the liquid phase. Biofilms at air-liquid and solid-air interfaces can cause serious problems in industrial water systems. In conclusion, our results indicate that IHF has an important regulatory role in biofilm formation of S. enterica serovar Enteritidis. Moreover, both IHF subunits appear to have a role in this process. Our data pave the way for further studies investigating the mechanisms involved in the regulation of biofilm formation by IHF. Acknowledgements This work was supported by grants from Fundaà §Ã £o de Amparo à Pesquisa do Estado de Sà £o Paulo (FAPESP 2014/13412-8) and Conselho Nacional de Desenvolvimento Cientà fico e Tecnolà ³gico (CNPq), Brazil. BL, DBN, and GPM were supported by a FAPESP fellowship (FAPESP 2012/25426-8, 2012/10608-3, and 2012/05382-6, respectively). CHW and CPC were supported by fellowships from CNPq (141629/2012-6 and 140786/2012-0, respectively). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest or conflict.
Friday, January 17, 2020
Dreams: Winnie-the-pooh and Vision Center Essay
We have prepared this handout of actual essays written by current Harvard students who attended secondary schools in the UK (with names changed for anonymity) in order to provide some guidance to schools and applicants. Because the university admissions processes in the US and the UK are markedly different, we have received requests for some sample essays and tips for writing them and hope they will be helpful. Here is the official description of the personal essay requirement: Please write an essay (250 words minimum) on a topic of your choice or on one of the options listed below. This personal essay helps us become acquainted with you as a person and student, apart from courses, grades, test scores, and other objective data. It will also demonstrate your ability to organize your thoughts and express yourself. 1. Evaluate a significant experience, achievement, risk you have taken, or ethical dilemma you have faced and its impact on you. 2. Discuss some issue of personal, local, national, or international concern and its importance to you. 3. Indicate a person who has had a significant influence on you, and describe that influence. 4. Describe a character in fiction, a historical figure, or a creative work (as in art, music, science, etc.) that has had an influence on you, and explain that influence. 5. A range of academic interests, personal perspectives, and life experiences adds much to the educational mix. Given your personal background, describe an experience that illustrates what you would bring to the diversity in a college community, or an encounter that demonstrated the importance of diversity to you. 6. Topic of your choice. Your essay for a US university might be the same one you would write for the UCAS system, but perhaps not. We are interested in your academic successes and future plans, but also want to understand what makes you tick as a person. What are your hopes, dreams and fears? Our advice is to think of two or three possible topics, write a quick first draft of each essay and then show them to your best friend, mother, teacher or anyone who knows you well. Ask that person if your voice and personality come through in the essays and which one sounds the most like you. Then take that essay and polish it off! As you will see from the following sample essays, these students have written about learning to ride a bike, culture shock at coming to the UK, music, public service, and a favourite book. What will you write about? Sample College Essay #1 I never imagined that by swimming, a Vision Center in India would be built. And I certainly never thought so many people could be cured of blindness there. For the past twelve years of my life, my passion has been competitive swimming. Mile after mile I train almost every single day in the hope of becoming that much faster, that much more powerful in the water, that much closer to my goals. (My classmates tell me I am better adapted to live in the water than on land!) I have reached more athletic goals than I ever imagined when I first splashed into the water as a timid six-year old. I have won several Texas state titles, been ranked nationally in both the US and the UK, set numerous International Schools Tournament records, and captained both my school and club swim teams. This past year, I decided to combine my love of swimming with a fundraising target. My older brother worked as an intern on the Flying Eye Hospital run by the international sight-saving ch arity ORBIS. I was horrified by his description of the magnitude of curable, but untreated eye diseases. I knew I had to take action. To help those who have or will lose their sight for no fault of their own, my triplet siblings and I organized, planned, publicized, and successfully led a community-wide Swim-a-thon that raised funds for ORBIS. The goal of our event was not only to raise funds for this very worthwhile cause, but more importantly, to raise awareness about avoidable blindness. Our theme ââ¬Å"Every minute a child goes blindâ⬠, caught the attention of the community. The word spread. People were surprised to know that we have the medical capability to cure millions of people with a simple surgery or eye droplets, yet hundreds of people lose their sight every day. As a two-year class vice president and student member of the Athletic Advisory Board, I was able to gain permission from the Head of School to plan the event. I convinced the Athletic Director to set aside pool time and recruited life guards. In order to garner support, I placed ads in the school newspaper, hung posters throughout my school, and persuaded my coach to replace an afternoon workout with the Swim-a-thon. After weeks of preparation, swim mates, school faculty, and parents logged thousands of laps. It was an immensely successful day. Enough money was collected to build a Vision Center in India, with surgical equipment, medicines, and training materials. The new Vision Center will not only treat thousands of patients, but will create a permanent site to train doctors and other medical personnel. As Treasurer of my schoolââ¬â¢s chapter of the National Honor Society, I plan to allocate charity funds this year to ORBIS for the continuing operational costs of the Vision Center. Every minute a child goes blind. Thirty-seven million people in the world are blind. Remarkably, an overwhelming 28 million of them do not need to be. When I think about the Vision Center we funded, I am overwhelmed with a sense of accomplishment and pride. Even though I will never meet the many people who will receive medical treatment there, the satisfaction of knowing that I have helped change the lives of thousands of people is astonishing. More meaningful than any swim race or trophy, we have brought hope where there was darkness. Sample College Essay #2 If we speak the same language, then why donââ¬â¢t I understand you? Why are the clothes you wear so different and the expressions you say so unclear to me? It was my first day in England and a ââ¬Å"Bank Holidayâ⬠at that. With only one sport on television, I was determined to watch and study a game I had no idea how to play. I didnââ¬â¢t know what an ââ¬Å"overâ⬠was, or even the job of the bowler. I didnââ¬â¢t know what a ââ¬Å"wicketâ⬠was, or how many a team needed to win. But I didnââ¬â¢t care. I was living across the pond now, and if I was going to fit into my new surroundings, understanding the rules of cricket seemed like a fine starting point. I persevered, and eventually I was explaining the now familiar game of cricket to my family; baseball analogies helped a great deal. I was proud of myself. I had conquered the English culture. Maybe England wasnââ¬â¢t so bad after all. I soon realized how naive these thoughts were. Cricket was just the beginning. A whole world of different traditions and customs was thrown in front of me. July Fourth was exchanged for Guy Fawkes Day and the ââ¬Å"the celebration with the turkeyâ⬠was erased from the calendar. Where would I fit in? Rugby and Premier League Football dominated the sports channels. Where was my beloved ESPN? Why is the television show, Little Britain, so hysterical? The movie theatres were smaller than a British mini cooper, and the Super Bowl kicked off at four a.m. The warmth of the Texan sun was replaced by the rainy days of Wimbledon. I was surprised to see that some parts of life abroad were better. Friends became mates. The frenetic pace of Piccadilly Circus and the splendour of St. Paulââ¬â¢s Cathedral are unsurpassed. Roundabouts make the traffic run smoothly. I like the sound of ââ¬Å"Cheersâ⬠. Over sixty different nationalities and over thirty languages are represented at the International School I attend. The culture shock was overwhelming. But I refused to yield. I was going to start by mixing into the English culture. I reported on local and national events as an Editor of the school newspaper. In addition I met swimmers from all over the U.K. through my British swim team, all with different backgrounds and lifestyles from mine. The cultures that engulfed me when I first came to England are part of me now. London is at my disposal. The people, the pubs, the expressions, and the entertainment are all a part of what makes living in England a once-in-a-lifetime experience. Yes, I have missed several Thanksgiving feasts and numerous Astros games, but I have come to understand and enjoy a completely new place. I wouldnââ¬â¢t change any of my experiences. Living in Europe has broadened my perspective on life and opened my eyes to so may wonderful people and ideas. People have similar goals wherever they come from. Iââ¬â¢m glad I know that there is no single right way to achieve them. Sample College Essay #3 I wake up and there is a rhythm in my head: itââ¬â¢s hazy. I climb into the shower and the water tapping on my scalp reminds me. As I sit on the bus to go to school, I get strange looks from passengers as I tap the rhythm onto my knees, but itââ¬â¢s not yet fully formed. Throughout the school day, I feel it evolve and develop until I inevitably sit down at my drums and play. From my brain via my heart it enters my muscles; they transfer it to the sticks which relay it to the drum. Eventually, the air gets my gift and the rhythm returns through my ears. Even after the sounds are gone, the rhythm is not. Until I go to bed, a dayââ¬â¢s repetition keeps it rebounding inside my cranium, in my own private concert hall. This is the journey of my daily rhythm. I wake up and there is a rhythm in my head: itââ¬â¢s not straight for this rhythm swings. At 6:00 am in Germany I get on a coach and as the wheels rotate beneath me I get closer. My coach has thirty five other people in it, each one is carried forward in their own sense of time, but in less than an hour they must all merge; seven hundred people will not accept a big band not keeping the pulse. Butterflies are roused in my gut and nerves take over. Iââ¬â¢ve never played a solo in front of so many people yet somehow my fear must be quelled. My imagination, my sticks and my drum-set have to communicate my inner rhythm; the audience must be able to feel it or else I have failed. Rhythm is the barrier to embarrassment. As the opening to ââ¬Å"Sing, Sing, Singâ⬠begins to take shape, all my trust is placed in the pattern I have within me transporting me safely to the end. If this vessel sank, I too would go with it. A standing ovation confirmed that this time, the barrier held strong. I wake up and there is a rhythm in my head: but it is quiet. In fact, no-one hears it; it makes no noise and never will. Between the hours of 0845 and 1545 I have 4 beats: each one signaling another unit of learning. This phrase is repeated 5 days a week for 40 weeks a year and the chorus goes on 6 more times. My song is my school, and in it I am caught up in its inner rhythms that I cannot control ââ¬â I must give in to them. The melodies that are assigned to these rhythms are made up of Virgil, esters and numbers that donââ¬â¢t exist. From these, cadences form that give me a chordal progression through education. Each part of my song has been given a name; there are no verses, no choruses but consecutive Key Stages. The rhythm indicates when I should make the transition: there is a series of fills, but they are not called fills. They call them exams and as the stages progress, the fills get more intense. In fact, they get more frequent and at the end of my school career, I look forward to a year where exams punctuate my calendar. In January, I will have moved to the dominant, only to complete the progression in June when I descend and finish on the tonic: a perfect cadence. I wake up and there is rhythm: the rhythm is life. The cycle of night and day and the constant pulsing in my chest are rhythms, and as the Earth revolves around our local star it is in time with the universe. I think in meter: a man crosses the street and his steps divide the distance between one curb and the next ââ¬â they provide a beat in the asphalt bar, or at least that is how I picture it. If animals could not use the rhythm of the seasons, then they would surely die. Life is a rhythm and all that it contains is in time. When the rhythm ceases to exist, so will I. Sample College Essay #4 Magicians are not truly magical, though they like others to think they are. So what inspires this ââ¬Å"deceptionâ⬠? Some think it is the money and others, the glamour of performing on stage and mystifying the audience. But for me it has always been a question of identity. Magic has helped me develop my confidence and communication skills so when the time comes to stand up and address a crowd, such as the school debate or a Model United Nations conference, it is no effort at all. However, I can say that one unusual circumstance in my life has given me a new sense of direction for my magic. At first, I thought magic was mere entertainment, but Horace, a man from the local Spastics Center diagnosed with autism in his late twenties, changed my perspective on my art. So often when we think of the disabled, we imagine children, and we sympathize with them. With Horace, I was faced with a situation largely unknown to the general publicââ¬â¢s experience: an unemotional adult who ra rely spoke to or acknowledged others around him. When I tried to engage him, he mumbled to me uninterested and somewhat detached. But then when I produced my deck of cards, when through several routines and then showed him how he, too, could create ââ¬Å"miraclesâ⬠, he smiled and laughed. This reaction highlighted the most rewarding aspect of magic because he accepted me into his world and responded to me. The Centerââ¬â¢s staff even commented, ââ¬Å"We have never seen Horace behaving in such an emotional way!â⬠For the first time, Horace had been given hope that he too could, perhaps, achieve and live a meaningful life. The magic had broken a myth of futility and dispelled it forever. I saw that magic could provide a driving force for pursuing change, and this realization overwhelmed me. I had witnessed something so unusual that the force of it took my breath away. The essence of magic is establishing a connection between the audience and the performer. With Horace, the ordinary had become the extraordinary, and for a moment, we connected in a state called ââ¬Å"Astonishmentâ⬠. This experience brings about a revisit to our most basic form, unaltered by culture or society. Indeed, that instant is so special because as adults, we are all too rarely astonished, and this moment returns us to our days as children when we were clueless and laughed at everything. This unusual encounter showed me that in this moment of astonishment, magic has the power to inspire. Since the encounter with Horace I have founded a society that brings all the magicians at my school together to perform magic for the elderly and the less fortunate in the nearby community. If I can show them, for instance, how to produce onepound coins from thin air, then contrary to what they have been told, perhaps they can challenge their ââ¬Å"limitsâ⬠. Then, dare I say that my passion for magic would be enhanced by a touch of true magic, generated perhaps from a truly unusual moment of astonishment. Sample College Essay #5 The ball ricocheted off the wall and disappeared into the black hole under my bed. It had been some years since the Hoover had been granted visiting rights and a heavy cloud of fluff covered every inch of the 4ââ¬â¢ x 6ââ¬â¢3â⬠rectangle. Slightly nervous of what I might find, I ventured in slowly with an outstretched hand. The ball was nowhere to be found but I felt a small box-shaped object. I dragged it out, dusted it off and there looking somewhat the worse for wear, was my old leather book trunk. As I eased open the lid, the familiar smell (slightly musty with time) transported me immediately back ten years. Inside, in pride of place on top, staring boldly back at me, was my old friend Winnie the Pooh. The familiar, faded yellow face, the shrunken red tshirt with tummy protruding proudly from beneath, an empty honey pot and by his side, as ever, was Piglet. It is to Winnie the Pooh that I owe my greatest debt. It was this funny bear of little brain and large appetite who first sparked my interest in the literary world. He taught me about friendships and Woozles and how to make the best Heffalump traps. Many a happy hour was spent with Pooh and his friends facing adversity with his ever optimistic demeanour. Although I havenââ¬â¢t seen this treasured copy for many a year, I must admit to a weekly dose of life in a Hundred Acre Wood. Each week I share my passion for reading with Class 2A at the local village primary school. We start off with one of Poohââ¬â¢s adventures ââ¬â richly embellished with different voices that perhaps A.A. Milne may not have intended but, nevertheless, seem to get the seal of approval from my six-year-old audiences. After this we get down to the nitty gritty ââ¬â the business of learning to read ââ¬â or as I like to call it ââ¬Å"Discovering How to Lose Yourselfâ⬠. I go round the class taking turns to listen to them read. Although the range of their abilities is surprisingly large they all make a huge effort and really enjoy themselves. Progress is made and more and more pupils get lost each week. All too soon it is time to go. I say my goodbyes and rush back to school for my next lesson. I hum a little happiness tune and as I round the corner into the school a large thundercloud looms above. In my head a very Pooh-like voice says ââ¬Å"Tut, tut, looks like rain!â⬠. Sample College Essay #6 Nothing of much significance ever happens on the Isle of Wight. And to those of you for whom island travels only involved the palm trees and pink sand variety, let me enlighten you. The Isle of Wight is a small, chalky lump that broke off the south coast of England and came to a halt one mile out. It is caught in a rather charming time warp ââ¬â circa. 1955. No palm tree could ever survive the freezing easterly winds that blast through from Siberia every winter and the only pink sand would be the result of a small child falling over a sandcastle on the way back from the Mr. Whippy ice cream van. It has sand and it has trees but of the altogether more hardy type. The sand is yellow and coarse, the trees are sturdy and solid with waxy leaves to withstand the salty air. The people are sturdy and solid too with an accent inherited from their pirate ancestors that sets them apart from those on the mainland. Life ambles along and nobody rushes. Pity anyone caught in a queue at the butchers behind Mrs. Singleton as she recounts, far too vividly, details of her latest health scare. Donââ¬â¢t get me wrong, it has its virtues ââ¬â the Victorian pier pointing like a lace gloved finger out to sea and the promenade with shops selling pink and white sticks of rock with ââ¬Å"Isle of Wightâ⬠running through it (how do they do that?), ââ¬Å"Kiss me Quickâ⬠sunhats and inflatable boats. And on the corner of the High Street, the Cod Father fish and chip shop with a sign in the window saying ââ¬Å"We batter anything!â⬠It was on this sleepy little island that I, as a small boy, spent most of my summers. Summers that, due to the temperature, would be called autumn anywhere else ââ¬â but nothing a long wetsuit and hat couldnââ¬â¢t disguise. One day in early July, arriving back from another bracing trip to the beach, I saw my mother and sister sitting side by side on the grassy bank overlooking the lawn. Nex t to them was a large bag of sweets and a bicycle. My mother announced that today was the day she was going to teach me how to ride a bike. There had been many attempts before but today I could tell she meant business. A succession of sweets was laid out at regular intervals along the lawn marking the route I was to take. Each time I made it to that point without falling off I got the sweet. Knowing that I really didnââ¬â¢t have any choice, I climbed awkwardly onto the enormous bike. Mother steadied me with one hand and then with a shove I was hurtling down the bank and onto the lawn. A blur of screaming and clapping preceded the first of many spectacular falls and my shins took on the blue hue of an uncooked lobster with blobs of grease from the chain splattered amongst the bumps. My sister, younger than me and already a whiz on her pink Barbie bike, gave patronizing advice trying to sound encouraging but, I suspect, rather enjoying the moment. An hour and a half later, battered and bruised with tears streaking through the mud on my face, I climbed onto the beast one more time. I steadied myself, focused on the hedge at the other end of the lawn (where the biggest chocolate bar lay) and with an almighty push I launched myself forward peddling with fury. Before I knew what had happened, there was a rush of green as I landed headfirst into the hedge. The bike, free of its incompetent rider, did an elegant ark to the left before coming to a halt. As I cycle confidently (but still canââ¬â¢t do the no hands thing) to the boathouse every day, I think of the words of Claude Pepper who said, ââ¬Å"Life is like riding a bicycle. You donââ¬â¢t fall off unless you plan to stop peddling.â⬠I wonââ¬â¢t stop peddling. ââ¬â and am proud to announce that on the 3rd July 1998 something of great significance did, at last, happen on the Isle of Wight.
Thursday, January 9, 2020
Term Paper about What is Platonic Love
Introduction The fact that the society has been involved in advancing feminism and gender equality, there is a fact that gender cannot ignore; no man is an island. The love and want of a soul mate keeps every man and woman searching for the ââ¬Ëother halfââ¬â¢. However, if this is necessary for the human race, why is it that the purpose and the meaning of it has eluded us? From the Platoââ¬â¢s symposium, in the epoch when the seeking for knowledge was as a pathway to enlightenment, the term love was a concept that was not well understood. This symposium serves as a pamphlet that shows the guidelines as seen by the philosophers at the time of Plato. The intervention of the Greek gods in their speeches is interpreted to mean different aspects of love and even their effects on the people. The symposium is a gathering of academically diverse and actually wise men who share same mind about love. The love between women and men is disparaged as lewd and procreant and the love between boys and men are praised as beneficial to wisdom. Main Discussion What is Real Love ââ¬â Plato on Love Plato postulates that, humans are made up of three planes: Body (Soma), Soul (Psiche) and the divine consciousness/ spirit (Nous). In all these three dimensions, humans search for eternity and completeness. This is because as divine creatures, humans would have an innate wish to go back to their original state i.e. the state of union with God. From that, it can be deduced that, love is force that drives man towards the original condition- the creator. From the physical plane, beauty is a key attribute of the divine. According to Plato, people have a natural tendency to consider beauty and even to search for beautiful bodies. Gorgeous forms instigate the humans to spread the seeds of eternity so as to give life to another being who will continue apiece of those who brought him to life. In a higher realm, the psychological plane, Man has a natural tendency to seek for the divine and should feel encouraged towards upright people so as to give birth to moral virtues and even to spread the seeds of eternity through building projects and also reaching objectives that will end up leaving traces of their existence. Eventually, in the highest realm, love seems to be the sentiment that rolls back everything back to unity. So, as Plato defended, real love is when oneà finds another one whom he/she canà manifest love in the three levels of existence and in addition feel fulfilled in all the dimensions i.e. Physiological, physical and the spiritual planes. Ideals of Love in Platos Symposium Plato considers love as a necessary in the life of man that enables him/ her to acquire joy and courage while living or dead. What is love? How does it feel like? How does it come about? These questions are difficult to explain, yet they are somehow understood. The Platoââ¬â¢s symposium, which dates back in the start of the middle period, Plato introduced his Eros theory (Eros usually was translated to mean love). This dinner party had the discussion of love as the main topic. The notion of love was understood at the end of the party came about gradually. This transformed from all speakers who spoke at the party and this was in comparison of the whole process of understanding the love that Socrates was trying to explain in his own speech. It is in the same symposium that Plato introduces his Eros theory. Fro these dialogues the western culture was also discussed- for instance the image of two lovers as being the others half, which Plato assigns to Aristophanes in the Symposium. Besides, the ââ¬Å"ladder of love,â⬠through which a lover can ascend to direct cognitive contact with beauty itself. The Phaedrus reveals love as being the great ââ¬Å"divine madnessâ⬠through which the wings of the loverââ¬â¢s soul may sprout, allowing the lover to take flight to all of the highest aspirations and achievements possible for humankind. In both these two cases, Plato regards sexual or physical contact between the lovers as not only wasteful but also degraded forms of erotic expression. Since the goal of love/ Eros is real beauty and that real beauty is the form of beauty (what Plato calls beauty itself) so eros only finds its fulfillment in Platonic philosophy. He further postulates that, unless it channels its lo ve power into what he calls higher pursuits, which result in the knowledge of the form of beauty, eros is subject to frustration. It is form this reason there for that Plato thinks that, many people squander the power of love by restricting themselves to the pleasures of the physical beauty. Why do People Suffer for Love? From this point of view, it is effortless to identify the reasons behind unhappy relationships. Humans focus on the physical dimension of love that is seeking for only the physical fulfillment and in turn neglecting the other aspects of psychological and the spiritual. Where a couple can give rise to off springs in the physical realm, but they cannot give rise to virtues together, or even to be united in a higher plane, then according to Plato this is not true love. This is simply satisfaction of the instincts, which is not only temporal but also fades away in the long run. Unluckily, many become aware of the spiritual and psychological dimensions of love when itââ¬â¢s too late and also when the two people have taken different bearings. In such at times the couple is united by merely the obligations of their physical existence but spiritually they are not together. Is Platonic Love Impossible? Soul mates are people (couple) who walk together towards the superlative world, they are spiritually, physically and psychologically together- in that, they complement each other in each of the three realms of existence. Some people think that platonic love is sexless, but rather, it is love that is not commanded by instincts, not ruled by the satisfactions of the body, but a kind of love that fulfills the spiritual needs and that brings forth not only off springs but also virtues to the world. In the contemporary society, people are used to love that is just based on the physical realm, that this form of love that transcends the frontiers of the earthily or material life is related to the impossible love. May be the contemporary society would have a lot to borrow from the ancient world. References Michael, J. Platonic love: Poems. New York: Orchises Press, 1991. Plato, C. Plato on love. New York: Hackett Publishing, 2006. Plato, Christopher, G. The symposium. New York: Penguine Classics, 2003. Price, A. Love and Friendship in Plato and Aristotle. London: Oxford University press, 1990. Campos, Thais. What is Platonic Love ââ¬â Platos Philosophy and the Art of Loving. 10 Aug 2010. 9 October 2010 http://www.suite101.com/content/what-is-platonic-loveplatos-philosophy-and-the-art-of-loving-a260700#ixzz12535H6gi.
Wednesday, January 1, 2020
The Leadership Styles Of The Executive Staff - 1383 Words
ââ¬Å"Was Enron the Work of a Few Bad Men or Dark Shadow of the American Dream?â⬠In August 2000, Enron, an American energy corporation, stock had reached a high of $90.75 per share. However, by November 2001, the price had plummeted to less than a dollar amidst the collapse of one of analystsââ¬â¢ most highly recommended investments. On December 2, 2001, Enron became the largest American corporate bankruptcy to date. The company was deceptive, even fooling Fortune Magazine into naming it ââ¬Å"Americaââ¬â¢s Most Innovative Companyâ⬠for six consecutive years. The leadership styles of the executive staff fostered a cutthroat and unethical business subculture for the climate which inevitably led to Enronââ¬â¢s demise. Two of Enronââ¬â¢s fearless leadersâ⬠¦show more contentâ⬠¦They were skilled communicators who developed a comprehensive and captivating vision for their company. They had referent power; their employees, stock analysts, and shareholders t rusted them. Analysts were blinded by the companyââ¬â¢s fraudulent practices because they took the financial statements from the management on faith. In ââ¬Å"Enron: The Smartest Guys in the Room,â⬠Amanda Martin, a top executive at the company, described Skilling saying, ââ¬Å"Jeff was like the prophet. He came in and said there s a whole new world out there...The excitement was palpable. You cannot imagine how proud we all were to be there, and then, of course, we had a leader who imbued us with a sense of confidence.â⬠Skilling controlled his employees using rational persuasion and inspirational appeals. He used logic but also appealed to their values, further blurring the line between the achievements of employees and the subsequent success of Enron. Skilling and Lay were clearly charismatic leaders but it was their lack of other leadership skills, including altruism and integrity, that exposed the dark potential of charismatic leaders. Lay and Skilling also were transactional leaders, ââ¬Å"leaders who guide or motivate their followers in the direction of established goals by clarifying role and task requirementsâ⬠(TB). They implemented strict work schedules to ensure that each employee was maximizing
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